Spatially selecting a single cell for lysis using light-induced electric fields.

نویسندگان

  • Christian Witte
  • Clemens Kremer
  • Mayuree Chanasakulniyom
  • Julien Reboud
  • Rab Wilson
  • Jonathan M Cooper
  • Steven L Neale
چکیده

An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.

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عنوان ژورنال:
  • Small

دوره 10 15  شماره 

صفحات  -

تاریخ انتشار 2014